来源:《Journal of Genetics and Genomics》2019年第05期  作者:Jingying Li;Jinman Luo;Meilian Xu;Shaoya Li;Jiahui Zhang;Huiyuan Li;Lei Yan;Yunde Zhao;Lanqin Xia;
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Plant genome editing using xCas9 with expanded PAM compatibility

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CRISPR/Cas enables robust genome editing and has revolutionized both functional genomics and crop breeding. The specificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA (sgRNA) and a short protospacer adjacent motif(PAM) in the genome (Cong et al., 2013; Zetsche et al., 2015). The(本文共计4页)......[继续阅读本文]

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